Affinity chromatography of rabbit liver .BETA.-glucuronidase.
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چکیده
منابع مشابه
Isolation and characterization of a chondroitin sulfate-degrading endo-beta-glucuronidase from rabbit liver.
An endo-beta-glucuronidase acting on chondroitin sulfate was isolated from rabbit liver and purified about 550-fold, using a combination of ammonium sulfate fractionation, DEAE-cellulose chromatography, gel filtration on Sephacryl S-300, affinity chromatography through heparin-Sepharose CL-6B and preparative polyacrylamide gel electrophoresis. The pH optimum of this enzyme was 4.0 and the Km va...
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Affinity chromatography is a technique used to purify compounds, such as proteins, that have the ability to non-covalently and reversibly bind specific molecules, known as ligands. This method differs from the classical chromatography techniques in that the protein is purified on the basis of a unique biochemical property. In affinity chromatography, the ligand is covalently attached to a matri...
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ژورنال
عنوان ژورنال: Chemical and Pharmaceutical Bulletin
سال: 1979
ISSN: 0009-2363,1347-5223
DOI: 10.1248/cpb.27.2212